Zero-order release of lysozyme from poly(ethylene glycol)/poly(butylene terephthalate) matrices

摘要: Protein release from a series of biodegradable poly(ether ester) multiblock copolymers, based on poly(ethylene glycol) (PEG) and poly(butylene terephthalate) (PBT) was investigated. Lysozyme-containing PEG/PBT films microspheres were prepared using an emulsion technique. Proteins effectively encapsulated dense polymer matrices formed. The swelling in water reached equilibrium within 3 days. degree increased with increasing PEG content molecular weight the segment. rate lysozyme could be tailored very precisely by controlling copolymer composition. Release rates ratio For block copolymers blocks 4000 g/mol, first-order observed. polymers segments 1000 600 profile followed near zero-order kinetics. A mathematical description mechanism developed which takes into account effect hydrolytic degradation solute diffusion. model found to consistent experimental observations. Finally, determination activity released protein showed that not damaged during formulation, storage periods.