Process for purifying a protein.
作者： Christopher A. Pargellis , Mark A. Staples
DOI:
关键词: Copper 、 Adsorption 、 Protein purification 、 Buffer (optical fiber) 、 Scientific method 、 Chemistry 、 Tris 、 Ligand (biochemistry) 、 Elution 、 Molecular biology 、 Chromatography
摘要: A process for the purification of proteins from solutions containing contaminants similar net charge and molecular weight is provided, comprising contacting a solution desired protein with an immobilized metal affinity chromatography resin in buffer low concentration weak ligand chelant resin. The adsorbed then eluted using having high same ligand, e.g., Tris. Particularly preferred features employ agarose-iminodiacetic acid resins copper cations are especially useful obtaining preparations homogeneous, stable rsT4 proteins.
lens.org 参考文章(21)
John E. McEntire, Ben W. Papermaster, Extraction and purification of biologically active lymphokines ,(1981)
Hsiang-Fu Kung, Susumu Honda, Hiromu Sugino, Immune interferon and a method for its extraction and purification ,(1984)
Rolf E. A. V. Axen, Jan P. E. Carlsson, Goran E. S. Lindgren, Hakan N. Y. Drevin, Reactive derivatives of HS-group-containing polymers ,(1978)
Kikuo Kotani, Tsuneo Okuyama, Tadashiro Fujii, Nobuaki Nakagawa, Disulfide compounds of a carrier, having S--S exchange reactivity ,(1979)
David Van Norman Goeddel, Roberto Crea, Polypeptides, process for their microbial production, intermediates therefor and compositions containing them ,(1981)
Patrick Hubert, Jerker Porath, Metal chelate affinity chromatography : I. Influence of various parameters on the retention of nucleotides and related compounds Journal of Chromatography A. ,vol. 198, pp. 247- 255 ,(1980) , 10.1016/S0021-9673(00)84764-0
Richard Henry Inacker, Raymond Whitney Sweet, Keith Charles Deen, Gail Marie Folena-Wasserman, Process for purification of soluble T4 ,(1988)
David V. Goeddel, Patrick W. Gray, Human immune interferon ,(1982)
Kenneth C. Olson, Rong-Chang Pai, Purification and activity assurance of precipitated heterologous proteins ,(1984)
D.C. Rijken, G. Wijngaards, M. Zaal-de Jong, J. Welbergen, Purification and partial characterization of plasminogen activator from human uterine tissue. Biochimica et Biophysica Acta. ,vol. 580, pp. 140- 153 ,(1979) , 10.1016/0005-2795(79)90205-8