Genome-guided analysis of physiological capacities of Tepidanaerobacter acetatoxydans provides insights into environmental adaptations and syntrophic acetate oxidation.

摘要: This paper describes the genome-based analysis of Tepidanaerobacter acetatoxydans strain Re1, a syntrophic acetate-oxidising bacterium (SAOB). Principal issues such as environmental adaptations, metabolic capacities, and energy conserving systems have been investigated potential consequences for acetate oxidation discussed. Briefly, in pure culture, T. grows with different organic compounds produces main product. In consortium hydrogenotrophic methanogen, it can also reverse its metabolism instead convert to formate/H2 CO2. It only proceed if product formed is continuously removed. process generates very small amount that scarcely enough growth, which makes this particular syntrophy special interest. As crucial member biogas-producing community ammonium-rich engineered AD processes, genomic features conferring ammonium resistance, bacterial defense, oxygen temperature tolerance were found, well attributes related biofilm formation flocculation. likely form an electrochemical gradient by putative electron-bifurcating Rnf complex [Fe-Fe] hydrogenases, observed other acetogens. However, deficiencies acetogenic anaerobic respiration discovered, lack formate dehydrogenase F1F0 ATP synthase. has pathways used under SAO non-SAO conditions. The two complete sets bacteriophage genomes, found be encoded genome, are worthy mention.