Trypanosoma cruzi: RNA structure and post-transcriptional control of tubulin gene expression.

摘要: Abstract Changes in tubulin expression are among the biochemical and morphological adaptations that occur during life cycle of Trypanosomatids. To investigate mechanism responsible for differential accumulation mRNAs Trypanosoma cruzi , we determine sequences α- β-tubulin transcripts analyzed their parasite. Two 1.9 2.3 kb were found to differ mainly by an additional 369 nucleotides at end 3 ′ untranslated region (UTR). Although transcription rates similar epimastigotes amastigotes, 3- 6-fold more abundant than trypomastigotes amastigotes. Accordingly, half-lives significantly higher Transient transfection experiments indicated positive regulatory elements UTR plus downstream intergenic α-tubulin gene both negative equivalent regions gene. Index Descriptions Abbreviations : Kinetoplastida; ; tubulin; regulation; PCR, polymerase chain reaction; UTR, region; IR, SL, spliced leader; BAC, bacterial artificial chromosome.