Depletion of a Drosophila homolog of yeast Sup35p disrupts spindle assembly, chromosome segregation, and cytokinesis during male meiosis

摘要: In the course of a genetic screen for male-sterile mutations in Drosophila affecting chromosome segregation during meiotic divisions spermatocytes, we identified mutation dsup35(63D). Examination mutant testes showed that misbehavior was consequence major disruptions spindle assembly. These perturbations included problems aster formation, separation, and migration around nuclear envelope; aberrations organization integrity; disappearance ana/telophase central spindle, which turn disrupts cytokinesis. The dsup35(63D) is caused by P element insertion affects, specifically testis, expression gene (dsup35) encoding homolog yeast Sup35p Xenopus eRF3 proteins. proteins are involved termination polypeptide synthesis on ribosomes, but previous studies have suggested closely related same family also interact directly with microtubules. An affinity-purified antibody directed against product dsup35 prepared; interestingly, this labels primary spermatocytes one or two discrete foci unknown structure within nucleoplasm. We discuss how depletion might lead to global assembly seen spermatocytes.