Characterization of neutral glycosphingolipids in human cataractous lens.
作者： M. Ogiso , A. Irie , H. Kubo , M. Komoto , T. Matsuno
DOI: 10.1016/S0021-9258(19)38643-0
关键词: Chromatography 、 Chemistry 、 Chromium trioxide 、 Globotriaosylceramide 、 Biochemistry 、 Glycolipid 、 Lactosylceramide 、 Metabolism 、 Ceramide 、 Sugar 、 Column chromatography
摘要: Neutral glycosphingolipids were purified from human senile cataractous lenses by a combination of solvent extraction, Folch's partition, acetylation, and column chromatography using DEAE-Sephadex Iatrobeads. Six major (A-F) monohexosylceramide to pentahexosylceramide identified sugar composition analysis, methylation secondary ion-mass spectrometry, glycosidase digestion, chromium trioxide oxidation. Their structures suggested that they closely related in their metabolism: chains sequence ceramide moieties similarly composed, namely C16:0 C24:1 constituted most the fatty acids, long-chain base components mostly C18-dihydrosphingosine with small portion C18-sphingosine. The implied two pathways branching lactosylceramide: one globotriaosylceramide other lactotriaosylceramide, which leads production Le(x) glycolipid via neolacto type 2 core chain.
sci-hub.st 参考文章(31)
M Komoto, M Ogiso, S Hirano, K Sudo, H Kubo, N Saito, Increase in lens gangliosides due to aging and cataract progression in human senile cataract. Investigative Ophthalmology & Visual Science. ,vol. 31, pp. 2171- 2179 ,(1990)
N Taniguchi, K Yanagisawa, A Makita, M Naiki, Purification and properties of rat liver globotriaosylceramide synthase, UDP-galactose:lactosylceramide alpha 1-4-galactosyltransferase. Journal of Biological Chemistry. ,vol. 260, pp. 4908- 4913 ,(1985) , 10.1016/S0021-9258(18)89158-X
Hung-ju Yang, Sen-itiroh Hakomori, A Sphingolipid Having a Novel Type of Ceramide and Lacto-N-Fucopentaose III Journal of Biological Chemistry. ,vol. 246, pp. 1192- 1200 ,(1971) , 10.1016/S0021-9258(19)76958-0
J C Steigerwald, S Basu, B Kaufman, S Roseman, Sialic acids. Enzymatic synthesis of Tay-Sachs ganglioside. Journal of Biological Chemistry. ,vol. 250, pp. 6727- 6734 ,(1975) , 10.1016/S0021-9258(19)40993-9
N. Kojima, S. Hakomori, Cell adhesion, spreading, and motility of GM3-expressing cells based on glycolipid-glycolipid interaction. Journal of Biological Chemistry. ,vol. 266, pp. 17552- 17558 ,(1991) , 10.1016/S0021-9258(19)47407-3
N Kojima, S Hakomori, Specific interaction between gangliotriaosylceramide (Gg3) and sialosyllactosylceramide (GM3) as a basis for specific cellular recognition between lymphoma and melanoma cells. Journal of Biological Chemistry. ,vol. 264, pp. 20159- 20162 ,(1989) , 10.1016/S0021-9258(19)47037-3
M Ogiso, A Irie, H Kubo, M Hoshi, M Komoto, Senile cataract-related accumulation of Lewis(x) glycolipid in human lens. Journal of Biological Chemistry. ,vol. 267, pp. 6467- 6470 ,(1992) , 10.1016/S0021-9258(19)50449-5
I Eggens, B Fenderson, T Toyokuni, B Dean, M Stroud, S Hakomori, Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells. Journal of Biological Chemistry. ,vol. 264, pp. 9476- 9484 ,(1989) , 10.1016/S0021-9258(18)60556-3
Reiji Kannagi, Kiyohiro Watanabe, Sen-Itiroh Hakomori, [1] Isolation and purification of glycosphingolipids by high-performance liquid chromatography Methods in Enzymology. ,vol. 138, pp. 3- 12 ,(1987) , 10.1016/0076-6879(87)38003-6
Atsushi Irie, Hideo Kubo, Fuyuhiko Inagaki, Motonori Hoshi, Ceramide dihexosides from the spermatozoa of the starfish, Asterias amurensis, consist of gentiobiosyl- cellobiosyl-, and lactosylceramide. Journal of Biochemistry. ,vol. 108, pp. 531- 536 ,(1990) , 10.1093/OXFORDJOURNALS.JBCHEM.A123237