Three-Dimensional in Vitro Model to Study Osteobiology and Osteopathology.

摘要: The bone is an amazing organ that grows and remodels itself over a lifetime. It generally accepted sculpting in response to stress force carried out by groups of cells contained within multicellular units are coordinated degrade existing form new bone. Because the nature extensiveness skeleton, it difficult study remodeling vivo. On other hand, because contains complex environment many cell types, possible vitro? We propose one can at minimum interaction between osteoblasts (bone formation) osteoclasts degradation) three dimensional (3D) “bioreactor”. Furthermore, add degrading metastatic cancer cells, how they contribute take part degradation process. have primarily cultured differentiated MC3T3-E1 for long periods (2–10 months) before addition marrow and/or (MDA-MB-231), metastasis suppressed (MDA-MB-231BRMS1) or non-metastatic (MCF-7) breast cells. In co-culture there was clear evidence matrix degradation. Loss also evident after with Tri–culture permitted evaluation types. 3D system holds promise further studies dormancy, hormone, cytokine effects manipulation. J. Cell. Biochem. 116: 2715–2723, 2015. © 2015 Wiley Periodicals, Inc.