Transplantation of Cryopreserved Adult Human Schwann Cells Enhances Axonal Conduction in Demyelinated Spinal Cord

摘要: Schwann cells derived from human sural nerve may provide a valuable source of tissue for cell-based therapy in multiple sclerosis. However, it is essential to show that transplanted can remyelinate axons adult CNS and improve axonal conduction. Sections were removed amputated legs patients with vascular disease or diabetes, isolated cryopreserved. Suspensions reconstituted into the X-irradiation/ethidium bromide lesioned dorsal columns immunosuppressed Wistar rat. After 3–5 weeks extensive remyelination, typical cell pattern was observed lesion zone. Many immunopositive an anti-human nuclei monoclonal antibody. The maintained vitro recording chamber; conduction properties studied using field potential intra-axonal techniques. displayed improved velocity frequency–response properties, action potentials conducted over greater distance lesion, suggesting block overcome. These data support conclusion transplantation results functional remyelination column lesion.