Isolation and characterization of a Paramecium cDNA clone encoding a putative serine/threonine protein kinase

摘要: Mitogen-activated protein (MAP) kinases, a closely related family of are involved in cell cycle regulation and differentiation yeast human cells. They have not been documented ciliates. We used PCR to amplify DNA sequences ciliated protozoan--Paramecium caudatum--using primers corresponding amino acid that common MAP kinases. isolated sequenced one putative kinase-like serine/threonine kinase cDNA from P. caudatum. This cDNA, called pcstk1 (Paramecium caudatum Serine/Threonine Kinase 1) shared approximately 35% identity with kinases yeast. activated by phosphorylation specific threonine tyrosine residues. These two residues conserved the PCSTK1 sequence at positions Thr 159 Tyr 161. The PSTAIRE motif, which is characteristic CDK2 gene family, cannot be found ORF PCSTK1. highest homology score was STK9, contains type domains. Comparisons expression level shown expressed equally cells different stages (sexual asexual). discussed possibility, as other organisms, genes exists