Cooperative control of holliday junction resolution and DNA repair by the SLX1 and MUS81-EME1 nucleases.
作者： Dennis Castor , Nidhi Nair , Anne-Cécile Déclais , Christophe Lachaud , Rachel Toth
DOI: 10.1016/J.MOLCEL.2013.08.036
关键词: Homologous recombination 、 DNA-binding protein 、 DNA repair 、 Cell biology 、 DNA 、 Recombinase 、 Biology 、 Holliday junction 、 Chromosome segregation 、 Genetics 、 MUS81
摘要: Holliday junctions (HJs) are X-shaped DNA structures that arise during homologous recombination, which must be removed to enable chromosome segregation. The SLX1 and MUS81-EME1 nucleases can both process HJs in vitro, they bind in close proximity on the SLX4 scaffold, hinting at possible cooperation. However, cellular roles of mammalian not yet known. Here, we use mouse genetics structure function analysis investigate function. Disrupting murine Slx1 Slx4 genes revealed essential for HJ resolution mitotic cells. Moreover, act together resolve a manner requires tethering SLX4. We also show SLX1, like MUS81-EME1, is required repair interstrand crosslinks, but this role appears independent cleavage, least These findings shed light mammals maintenance genome stability.
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