IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions.

摘要: EndoS from Streptococcus pyogenes efficiently hydrolyzes the functionally important and conserved N-linked glycan of IgG in human blood. Repeated i.v. administration rabbits completely glycans whole pool, despite generation anti-EndoS antibodies. had no apparent effects on health animals. hydrolysis has profound effector functions, such as complement activation Fc receptor binding, suggesting that enzyme could be used an immunomodulatory therapeutic agent against IgG-mediated diseases. We demonstrate here indeed a protective effect mouse model lethal IgG-driven immune (or idiopathic) thrombocytopenic purpura. pretreatment pathogenic antibodies inhibits development disease, also rescues mice already established disease when severe thrombocytopenia s.c. bleeding have developed. These results identify potential diseases where are further emphasize antibody possible targets future therapies antibody-mediated autoimmune conditions.