A facile in vivo procedure to analyze metabolic pathways in intact lichens
作者： Veronika Kuhn , Thomas Geisberger , Claudia Huber , Andreas Beck , Wolfgang Eisenreich
DOI: 10.1111/NPH.15968
关键词: Polyketide 、 Biochemistry 、 Lichen 、 Usnic acid 、 Biosynthesis 、 Metabolic pathway 、 Thallus 、 Usnea 、 Chemistry 、 Amino acid
摘要: Lichen secondary metabolites show important biological activities as well pharmaceutical and chemotaxonomic potential. In order to utilize such substances of interest, detailed knowledge their biosynthetic pathways is essential. 13 CO2 -pulse/chase experiments using intact thalli the lichen Usnea dasopoga resulted in multiple C-labeled isotopologs amino acids, but not dibenzofuran derivative usnic acid - one best-studied metabolites, with considerable renewed interest for lifestyle applications. Spraying an aqueous solution [U-13 C6 ]glucose onto U. dasopoga afforded a specific mixture acid. One- two-dimensional NMR analysis crude extract corroborated polyketide pathway via methylphloroacetophenone phloroacetophenone. With exemplar, we provide proof-of-principle that can be used general study metabolic fluxes lichens.
sci-hub.se 参考文章(64)
Rosmarie Honegger, Lichen-forming fungi and their photobionts The Mycota. pp. 307- 333 ,(2009) , 10.1007/978-3-540-87407-2_16
Y Radha Krishna, V Mittal, P Grewal, MI Fiel, T Schiano, Acute liver failure caused by 'fat burners' and dietary supplements: a case report and literature review. Canadian Journal of Gastroenterology & Hepatology. ,vol. 25, pp. 157- 160 ,(2011) , 10.1155/2011/174978
Moreno Cocchietto, Nicola Skert, Pier Nimis, Gianni Sava, A review on usnic acid, an interesting natural compound. Naturwissenschaften. ,vol. 89, pp. 137- 146 ,(2002) , 10.1007/S00114-002-0305-3
A.M. Al-Bekairi, S. Qureshi, M.A. Chaudhry, D.R. Krishna, A.H. Shah, Mitodepressive, clastogenic and biochemical effects of (+)-usnic acid in mice. Journal of Ethnopharmacology. ,vol. 33, pp. 217- 220 ,(1991) , 10.1016/0378-8741(91)90079-S
Joanne G. Romagni, Giovanni Meazza, N.P.Dhammika Nanayakkara, Franck E. Dayan, The phytotoxic lichen metabolite, usnic acid, is a potent inhibitor of plant p-hydroxyphenylpyruvate dioxygenase. FEBS Letters. ,vol. 480, pp. 301- 305 ,(2000) , 10.1016/S0014-5793(00)01907-4
Werner Römisch-Margl, Nicholas Schramek, Tanja Radykewicz, Christian Ettenhuber, Eva Eylert, Claudia Huber, Lilla Römisch-Margl, Christine Schwarz, Maria Dobner, Norbert Demmel, Bernhard Winzenhörlein, Adelbert Bacher, Wolfgang Eisenreich, 13CO2 as a universal metabolic tracer in isotopologue perturbation experiments. Phytochemistry. ,vol. 68, pp. 2273- 2289 ,(2007) , 10.1016/J.PHYTOCHEM.2007.03.034
Heihachiro Taguchi, Ushio Sankawa, Shoji Shibata, Biosynthesis of usnic acid in lichens Tetrahedron Letters. ,vol. 7, pp. 5211- 5214 ,(1966) , 10.1016/S0040-4039(01)89308-4
Wolfgang Eisenreich, Nihat Knispel, Andreas Beck, Advanced methods for the study of the chemistry and the metabolism of lichens Phytochemistry Reviews. ,vol. 10, pp. 445- 456 ,(2011) , 10.1007/S11101-011-9215-3
Emi Okuyama, Kazuhiro Umeyama, Mikio Yamazaki, Yasuhiro Kinoshita, Yoshikazu Yamamoto, Usnic acid and diffractaic acid as analgesic and antipyretic components of Usnea diffracta. Planta Medica. ,vol. 61, pp. 113- 115 ,(1995) , 10.1055/S-2006-958027
Peter A. Cohen, G.H. Neil Towers, Biosynthetic studies on chlorinated anthraquinones in the lichen Nephroma laevigatum Phytochemistry. ,vol. 42, pp. 1325- 1329 ,(1996) , 10.1016/0031-9422(96)00148-3