A novel mouse model of red blood cell storage and posttransfusion in vivo survival

摘要: BACKGROUND: Storage of red blood cells (RBCs) is necessary for an adequate supply. However, reports have identified potential negative sequelae transfusing stored RBCs. An animal model would be useful to investigate the pathophysiology it has been reported that storage rat RBCs in CPDA-1 resulted unexpected sudden decline posttransfusion survival. A mouse RBC and transfusion was developed assess survival kinetics RBCs. STUDY DESIGN AND METHODS: expressing green fluorescent protein were collected CPDA-1, filter leukoreduced, adjusted a 75% hematocrit, at 4°C. At weekly intervals, transfused into C57BL/6 recipients. measured by flow cytometry chromium-51 labeling. Phosphatidylserine externalization CD47 expression also evaluated. RESULTS: Mean 24-hour survivals 99, 91, 64, 54, 30, 18% after 0, 7, 14, 21, 28, 35 days storage, respectively. Stored showed initial rapid clearance with subsequent extended Increased surface phosphatidylserine decreased observed. CONCLUSIONS: Mouse progressive survival, as function time, unlike precipitous loss viability Moreover, changes markers analogous trends human Together, these findings provide characterization novel serve experimental platform studying pathophysiologic consequences