The Frequency of Phosphorylcholine-Specific B Cells in Conventional and Germfree Balb/c Mice

摘要: The frequency of B cells specific for phosphorylcholine (PC) in spleens nonimmune conventionally-reared and germfree adult Balb/c mice was determined by an vitro splenic focus technique. Analysis spleen from 11 conventionally reared donors revealed average 19.1 PC-specific clonal precursor cells/10 6 donor when calculated utilizing a cloning efficiency previously the technique 4%. This number is one-tenth 2,4-dinitrophenyl- 2,4,6-trinitrophenyl-specific cells; however, both idiotypic isoelectric analysis reveal that majority PC yield monoclonal antibodies which share TEPC 15 idiotype contain homogeneous IgM peak with pK 4.60. Thus this single clone appears on to be represented more than 10 3 adults. A similar clonotype found seven so high independent previous antigenic stimulation. Among analyzed, frequencies varied 1.6 52/10 cells—an extreme degree variation compared almost invariant observed 2,4-dinitrophenyl, 2,4,6-trinitrophenyl, fluoroscein. interpreted as reflection life cycle possible role antigen implied smaller variations mice.