Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p

摘要: Therapeutic strategies for targeting angiogenesis have been proven as successful treatments divergent cancers. We previously discovered an anti-angiogenic miR-542-3p, which directly targeted the key angiogenesis-promoting protein Angiopoietin-2 to inhibit tumor in breast cancer models. In this study, further investigate mechanism of miR-542-3p induced angiogenic inhibition, we screened cell derived factors were responsible alteration endothelial cells. found that cell-derived angiogenin downregulated Overexpression cells facilitated activation both vitro and vivo models via inhibition miR-542-3p. Furthermore, our results showed could suppress CEBPB POU2F1, transcription suggesting a novel cell-endothelial signal pathway. addition, level primary carcinomas correlated with clinical progression. Serum levels associated metastatic development patients. Together, these findings reveal regulatory pathway whereby tumor-derived activates through may represent promising target therapy potential marker monitoring disease