Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.

作者: Carla Fenoglio , Livia Visai , Concetta Addario , Giuseppe Gerzeli , Gloria Milanesi

DOI: 10.1016/J.YGCEN.2004.03.008

关键词: KidneyParacrine signallingBiologyMesonephrosNitric oxide synthaseNitric oxideNicotinamide adenine dinucleotide phosphateBiochemistryGuanylate cyclase activityEnzyme assay

摘要: Natriuretic peptides (NPs), a family of structurally related hormones and nitric oxide (NO), generated by synthase (NOS), are believed to be involved in the regulation fluid balance sodium homeostasis. Differential expression these factors depend on both physiological pathological conditions. Both NPs NO act target organs through activation guanylate cyclase (GC) generation guanosine 3',5'-cyclic monophosphate (cGMP), which is considered common messenger for action factors. The present study was designed investigate--by histochemical methods--the some (proANP ANP) isoforms NOS (neuronal NOS, nNOS, inducible iNOS) mesonephros Rana esculenta different periods year including hibernation, evaluate possible seasonal changes their expression. We also studied enzyme activity NOS-related nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) GC. experiments were performed pieces kidney R. collected natural environment during active hibernating life. carried out using immunohistochemical techniques demonstrate proANP, ANP, isoforms. Antigen capture linked immunosorbent assay (ELISA) determine presence frog extract. Enzyme histochemistry used NADPHd at light microscopy; GC visualized electron microscope, cerium as agent. application demonstrated that tubules express patterns distribution and/or ANP annual cycle. Comparing results obtained frogs has provided interesting data; NOS/NADPHd activities showed variations well. Furthermore, extract evidenced dose-dependent response ELISA. data suggest intra-renal paracrine autocrine may modulate adaptations renal functions cGMP from activity.

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