Soluble IL-2 receptor in AIDS. Correlation of its serum level with the classification of HIV-induced diseases and its characterization.

摘要: By using a fluorescence sandwich ELISA, elevated IL-2R levels were detected in the sera from both HIV-infected hemophiliacs and other patients. The serum reflective of classification HIV-induced diseases by Centers for Disease Control. Moreover, negatively correlated most prominently with CD4 cell counts, lymphocyte decrease CD4-CD8 ratio but not either WBC counts or B counts. As striking elevations noted AIDS patients group IVD infection, was purified sequentially size-exclusion HPLC, high-pressure chromatofocusing, H48 affinity HPLC. isoelectric point values separated into 4.2 3.8, whereas Mr determined to be only 45 kDa immunoprecipitation antibody followed SDS-PAGE. However, production cellular supernatant PBMC any 19 HIV-I- HIV-II-infected line cells. In contrast, adult T leukemia cells that expressed human lymphotropic virus -I -II produced soluble IL-2R, constitutively. mechanisms which might are discussed comparison