Fasting activated histaminergic neurons and enhanced arousal effect of caffeine in mice

摘要: Abstract Caffeine, a popular psychoactive compound, promotes wakefulness via blocking adenosine A 2A receptors in the shell of nucleus accumbens, which projects to arousal histaminergic tuberomammillary (TMN). The TMN controls several behaviors such as and feeding. Fasting has been reported activate neurons increase arousal. Therefore, we propose that caffeine may promote greater under fasting rather than normal feeding conditions. In current study, locomotor activity recording, electroencephalogram (EEG) electromyogram recording c-Fos expression were used wild type (WT) histamine H 1 receptor (H R) knockout (KO) mice investigate effects Caffeine (15 mg/kg) enhanced fasted for 5 h, but only did so 3 h normally fed animals. Pretreatment with R antagonist pyrilamine abolished caffeine-induced stimulation on mice. EEG recordings confirmed WT mice, 5 h ones. stimulatory effect was not observed KO Furthermore, increased These results indicate had wakefulness-promoting through mediation R.