A specific βγ-subunit of transducin stimulates ADP-ribosylation of the α-subunit by pertussis toxin
作者： Hiroshi Ohguro , Yoshitaka Fukada , Tôru Yoshizawa , Tetsuya Saito , Toyoaki Akino
DOI: 10.1016/0006-291X(90)90656-8
关键词: ADP-ribosylation 、 Toxin 、 Visual phototransduction 、 Biochemistry 、 Transducin 、 Pertussis toxin 、 Binding protein 、 Stimulation 、 Protein subunit 、 Cell biology 、 Chemistry 、 Biophysics 、 Molecular biology
摘要: Abstract Transductin (Tα and Tβγ) is a GTP-binding protein involved in the visual transduction process rod outer segment. We have previously demonstrated that Tβγ mixture composed of two components, Tβγ-1 Tβγ-2, with distinctive γ-subunits, Tγ-1 Tγ-2, respectively (Fukada et al., 1989, J. Biol. Chem. , 264 5937–5943). To investigate interaction between Tα components Tβγ, effect either or Tβγ-2 on ADP-ribosylation catalyzed by pertussis toxin (IAP) was examined. stimulated IAP, while displayed almost no enhancement ADP-ribosylation. Addition to had Tα. These results indicate form complex serves as substrate IAP reaction, has little affinity for It suggested Tγ-2 an essential subunit Tβ interact
elsevier.com
sci-hub.se 参考文章(10)
S E Navon, B K Fung, Characterization of transducin from bovine retinal rod outer segments. Participation of the amino-terminal region of T alpha in subunit interaction. Journal of Biological Chemistry. ,vol. 262, pp. 15746- 15751 ,(1987) , 10.1016/S0021-9258(18)47791-5
Y Fukada, H Ohguro, T Saito, T Yoshizawa, T Akino, βγ-Subunit of bovine transducin composed of two components with distinctive γ-subunits Journal of Biological Chemistry. ,vol. 264, pp. 5937- 5943 ,(1989) , 10.1016/S0021-9258(18)83640-7
V N Hingorani, D T Tobias, J T Henderson, Y K Ho, Chemical cross-linking of bovine retinal transducin and cGMP phosphodiesterase. Journal of Biological Chemistry. ,vol. 263, pp. 6916- 6926 ,(1988) , 10.1016/S0021-9258(18)68731-9
Hermann Kühn, Light- and GTP-regulated interaction of GTPase and other proteins with bovine photoreceptor membranes. Nature. ,vol. 283, pp. 587- 589 ,(1980) , 10.1038/283587A0
Douglas R. Lowy, Berthe M. Willumsen, New clue to Ras lipid glue Nature. ,vol. 341, pp. 384- 385 ,(1989) , 10.1038/341384A0
Evgeniy E. Fesenko, Stanislav S. Kolesnikov, Arkadiy L. Lyubarsky, Induction by cyclic GMP of cationic conductance in plasma membrane of retinal rod outer segment Nature. ,vol. 313, pp. 310- 313 ,(1985) , 10.1038/313310A0
Patrick J. Casey, Michael P. Graziano, Alfred G. Gilman, G protein .beta..gamma. subunits from bovine brain and retina: equivalent catalytic support of ADP-ribosylation of .alpha. subunits by pertussis toxin but differential interactions with Gs.alpha. Biochemistry. ,vol. 28, pp. 611- 616 ,(1989) , 10.1021/BI00428A029
Yoshitaka Fukada, Toshiyuki Okano, Igor D. Artamonov, Tôru Yoshizawa, Chicken red-sensitive cone visual pigment retains a binding domain for transducin FEBS Letters. ,vol. 246, pp. 69- 72 ,(1989) , 10.1016/0014-5793(89)80255-8
André Bensadoun, David Weinstein, Assay of proteins in the presence of interfering materials. Analytical Biochemistry. ,vol. 70, pp. 241- 250 ,(1976) , 10.1016/S0003-2697(76)80064-4
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0