Factor B is essential for ATP synthesis by mitochondria.
作者： Grigory I Belogrudov
DOI: 10.1016/S0003-9861(02)00431-9
关键词: Oxidative phosphorylation 、 Photophosphorylation 、 ATP synthase 、 Biochemistry 、 Submitochondrial particle 、 Chemiosmosis 、 Complement factor B 、 Biology 、 ATP synthase gamma subunit 、 ATP synthase alpha/beta subunits
摘要: Factor B is a water-soluble protein, which required for the coupled activity of mitochondrial ATP synthase complex. Specific removal factor from well-coupled bovine heart submitochondrial particles (SMP) results in uncoupling and loss ATP-driven membrane potential formation reverse electron transfer succinate to NAD. Addition recombinant human (molecular mass 20,341 Da) B-depleted SMP (AE-SMP) restores these properties [G.I. Belogrudov, Y. Hatefi, (2002) J. Biol. Chem. 277, 6097-6103]. This paper shows that extraction purification complex (complex V) mitochondria extensive B. AE-SMP completely lost oxidative phosphorylation ATP-32P(i) exchange activities increases V by 2.5-fold. These further indicate an essential component mammalian
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