Synthetic furanones inhibit quorum-sensing and enhance bacterial clearance in Pseudomonas aeruginosa lung infection in mice.

摘要: Introduction: Antibiotics are used to treat bacterial infections by killing the bacteria or inhibiting their growth, but resistance antibiotics can develop readily. The discovery that quorum-sensing regulates virulence as well formation of biofilms opens up new ways control certain infections. Furanone compounds capable systems have been isolated from marine macro alga Delisea pulchra. Objectives: Two synthetic furanones were tested for ability attenuate in mouse models chronic lung infection targeting without directly growth. Methods: Study I. Mice with Escherichia coli MT102 [luxR-Pluxl-gfp(ASV)] injected intravenously N-acyl homoserine lactones test interference quorum-sensing. II. Pseudomonas aeruginosa PAO1 [dsred, lasR-PlasB-gfp(ASV)] evaluate effects on III. P. treated different doses therapeutic infection. Results: Furanones successfully interfered lactone and suppressed lungs, which resulted decreases expression green fluorescent protein. accelerated clearance, reduced severity pathology. In a lethal infection, treatment furanone significantly prolonged survival time mice. Conclusion: Synthetic inhibited exhibited favourable