Assessment of the Efficacy of a Constructed Wetland to Reduce or Remove Wastewater Effluent Estrogenicity and Toxicity Using Biomarkers in Male Fathead Minnows (Pimephales Promelas Rafinesque, 1820)

摘要: Vitellogenin in Pimephales promelas was used to assess estrogenicity of a local municipal effluent. induction male P. increased frequency and magnitude with exposure duration greater ("=0.05) than controls after 2 3 weeks exposure. The level vitellogenesis induced by effluent high compared similar studies. A spring season evaluation followed. Biomarkers were the efficacy treatment wetland remove toxicity final treated wastewater Comparisons made an dominated stream laboratory controls. Vitellogenin, GSIs (gonado-somatic indices), HSIs (hepato-somatic indices) secondary sexual characteristics biomarkers models aqueous estrogenicity. Biological indicators general fish health included hematocrit condition factors. estrogenic nature screened, concurrent exposure, GC/MS analysis for target compounds including: 17-b estradiol, estrone, ethynylestradiol, Bisphenol A, nonylphenolic compounds, phthalates, DDT. Plasma vitellogenin measured significantly elevated (p < 0.0001) at inflow site sites. these exposures less (a=0.001) site. At sites closest inflow, charateristics, tubercle numbers fat pad thickness, (a=0.0001). Hematocrit factors closer inflow. Seasonal variation examined repeating characterization summer. Additionally, summer testing dilution series. Fish heavily influenced interpretation results. Pre-acclimation spawning stresses may have altered many biological markers measured. Results are discussed relative pre-exposure environment. Toxicity assessed Ceriodaphnia dubia Vibrio fischeri toxicty tests on this somewhat sensitive C. test endpoints, but more V. fisheri.