Applications of diagonal chromatography for proteome-wide characterization of protein modifications and activity-based analyses

摘要: Numerous gel-free proteomics techniques have been reported over the past few years, introducing a move from proteins to peptides as bits of information in qualitative and quantitative proteome studies. Many shotgun randomly sample thousands manner but overlook vast majority protein modifications that are often crucial for proper structure function. Peptide-based proteomic approaches thus developed profile diverse set including, not at all limited, phosphorylation, glycosylation ubiquitination. Typical here is each modification needs specific, tailor-made analytical procedure. In this minireview, we discuss how one technique - diagonal reverse-phase chromatography applied study two different types modification: processing N-glycosylation. Additionally, an activity-based which purine-binding were profiled by chromatography.