Methods of Study

摘要: Man’s knowledge concerning the blood-brain barrier has exploded during past 25 years due in part to development of a vast array methods examine characteristics and transport properties both vivo vitro. Barrier permeability uptake have been studied using diverse series peripheral injection, single pass extraction, brain perfusion techniques, which established many basic principles physiology. structure function probed at morphologic level with electron microscopy immunohistochemistry. Quantitative autoradiography morphometry that regions differ solute transfer differences capillary density possibly function. Questions arisen extent all capillaries are perfused, whether perfused fraction is regulated meet needs cerebral metabolism. In few years, techniques cellular molecular biology developed culture endothelia, identify isolate specific carriers, study factors control expression Such studies may, future, genes define barrier, thereby allowing physicians some future date selectively “turn on” or off” functions aid clinical therapy.