A simplification of Heukeshoven and Dernick's silver staining of proteins
作者： Catherine Damerval , Martine Le Guilloux , Joël Blaisonneau , Dominique de Vienne
关键词: Silver nitrate 、 Distilled water 、 Sodium carbonate 、 Polyacrylamide 、 Silver stain 、 Chemistry 、 Chromatography
摘要: The procedure of Heukeshoven and Dernick for silver staining proteins in polyacrylamide gels is simplified by applying Farmer's reducer before impregnation with nitrate rinsing the gel 2.5 % sodium carbonate instead distilled water. This modification yields pattern good contrast without a destaining-restaining step.
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