Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.

摘要: Photoactivated genetically encoded voltage indicators (GEVIs) have the potential to enable optically sectioned imaging at intersection of a photoactivation beam and an beam. We developed pooled high-throughput screen identify archaerhodopsin mutants with enhanced photoactivation. After screening ~105 cells, we identified novel GEVI, NovArch, whose one-photon near-infrared fluorescence is reversibly by weak blue or two-photon excitation. Because leads fluorescent signals catalytically rather than stoichiometrically, high signals, optical sectioning, time resolution are achieved simultaneously modest laser power. demonstrate applications combined molecular tools mapping membrane in distal dendrites acute mouse brain slices spontaneously active neurons vivo.