Nuclear transplantation in mouse embryos.

摘要: The ability of foreign nuclei to support development in nuclear transplantation manipulations has proven an effective means assess the consequences differentiation. In addition, might serve define persistence and role maternally inherited cytoplasmic constituents during embryogenesis. We have extended use a technique that enables efficient transfer one-cell-stage pronuclei into cytoplasm enucleated mouse embryos, successfully transferred two-, four-, eight-cell-stage inner cell mass (ICM) nuclei. also used this as determining stage-specific embryonic antigen, SSEA-3, is contribution unfertilized ovum. potential value developmental capacity from various states, nuclear/cytoplasmic origins or early gene products, discussed.