Overexpressed lncRNA AC068039.4 Contributes to Proliferation and Cell Cycle Progression of Pulmonary Artery Smooth Muscle Cells Via Sponging miR-26a-5p/TRPC6 in Hypoxic Pulmonary Arterial Hypertension.
作者： Yuhan Qin , Boqian Zhu , Linqing Li , Dong Wang , Yong Qiao
DOI: 10.1097/SHK.0000000000001606
关键词: Hypoxia (medical) 、 microRNA 、 Medicine 、 Cancer research 、 Pulmonary artery 、 Pulmonary hypertension 、 Downregulation and upregulation 、 G1 phase 、 Microarray analysis techniques 、 Real-time polymerase chain reaction
摘要: Background Hypoxic pulmonary hypertension (HPH) is a devastating and incurable disease characterized by vascular remodeling, resulting in right heart failure even death. Accumulated evidence has confirmed long coding RNAs (lncRNAs) are involved hypoxia-induced remodeling HPH. The exact mechanism of lncRNA hypoxic remains unclear. Methods Microarray analysis was applied to investigate the profiles expression artery smooth muscle cells (PASMCs) cultured under hypoxia normoxia condition. qRT-PCR performed for lncRNAs, miRNA, mRNAs, western blot employed detection proteins. CCK-8 transwell chamber assay were assessment PASMC proliferation migration, respectively. Besides, flow cytometry assessments cell cycle progression. binding between AC068039.4 miR-26a-5p, TRPC6 3'UTR detected dual luciferase reporter assay. Results A total 1,211 lncRNAs (698 up-regulated 513 down-regulated) differently expressed PASMCs. Consistent with microarray analysis, quantitative PCR verified that obviously Knocking down alleviated migration PASMCs regulated progression through inhibiting entering G0/G1 phase. Further experiment indicated promoted via sponging miR-26-5p. In addition, transient receptor potential canonical 6 (TRPC6) be target gene miR-26a-5p. Conclusion conclusion, downregulation inhibited AC068039.4/miR-26a-5p/TRPC6 axis, providing new therapeutic insights treatment
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