Characterization by next-generation sequencing of 24 new microsatellite loci for the barred sand-bass, Paralabrax nebulifer (Girard, 1854), from the Baja California Peninsula, Mexico

摘要: We characterized a set of new hypervariable microsatellite loci for the barred sand-bass (Paralabrax nebulifer), marine fish that supports important recreational and artisanal fisheries in California, USA west coast Baja California Peninsula, Mexico. performed shotgun genome sequencing with 454 XL titanium chemistry used bioinformatics to search perfect repeats. selected 40 primer pairs were synthesized genotyped an ABI PRISM 3730XL DNA sequencer 32 individuals from San Juanico, Sur. estimated levels genetic diversity, deviations linkage Hardy–Weinberg equilibrium, frequency null alleles probability individual identity markers. successfully scored 24 (13 tetranucleotides 11 dinucleotides). The average number per locus was 12.5 (range 4–23). observed expected heterozygosities 0.779 0.313–0.969) 0.774 0.350–0.939), respectively. detected significant disequilibrium two loci. Genotype frequencies at seven showed expectations equilibrium had allele ≥10%. 8.5−36. markers will be useful investigating patterns fine-scale structure diversity estimate larval dispersal assess metapopulation dynamics, information necessary sustainable management P. nebulifer Peninsula.