A histone deacetylase, MoHOS2 regulates asexual development and virulence in the rice blast fungus.

摘要: Histone acetylation/deacetylation represent a general and efficient epigenetic mechanism through which fungal cells control gene expression. Here we report developmental requirement of MoHOS2-mediated histone deacetylation (HDAC) for the rice blast fungus, Magnaporthe oryzae. Structural similarity nuclear localization indicated that MoHOS2 is an ortholog Saccharomyces cerevisiae Hos2, member class I deacetylases subunit Set3 complex. Deletion led to 25% reduction in HDAC activity, compared wild-type, confirming it bona-fide HDAC. Lack caused decrease radial growth impinged dramatically on asexual sporulation. Such activity phenotypic defects ΔMohos2 were recapitulated by single amino acid change conserved motif known be important activity. Expression analysis revealed up-regulation concomitant down-regulation some key genes involved reproduction under sporulation-promoting condition. In addition, deletion mutant exhibited defect appressorium formation from both germ tube tip hyphae. As result, was not able cause disease symptoms. Wound-inoculation showed compromised its ability grow inside host plants as well. We found ROS detoxifying effector are de-regulated mutant. Taken together, our data suggest MoHOS2-dependent pivotal proper timing induction transcription coordinate changes infection M.