Optoribogenetic control of regulatory RNA molecules
作者： Sebastian Pilsl , Charles Morgan , Moujab Choukeife , Andreas Möglich , Günter Mayer
DOI: 10.1101/2020.07.07.191379
关键词: Gene expression 、 Cell state 、 Function (biology) 、 Cell biology 、 Protein function 、 Regulatory rna 、 Chemistry
摘要: Short regulatory RNA molecules underpin gene expression and govern cellular state physiology. To establish a novel layer of control over these processes, we generated chimeric RNAs that interact reversibly light-dependently with the light-oxygen-voltage photoreceptor PAL. By harnessing this interaction, function micro (miRs) short hairpin (sh) in mammalian cells can be regulated spatiotemporally precise manner. The underlying strategy is generic adapted to near-arbitrary target sequences. Owing full genetic encodability, it establishes unprecedented optoribogenetic cell method stands facilitate non-invasive, reversible resolved study protein organismal environments.
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