Regulation of lymphocyte calcitonin receptors by interleukin-1 and interleukin-6.
作者： J. J. Body , G. Fernandez , M. Lacroix , P. Vandenbussche , J. Content
DOI: 10.1007/BF00297185
关键词: Lymphocyte 、 Endocrinology 、 Bone resorption 、 Calcitonin 、 Receptor 、 Binding site 、 Biology 、 Interleukin 、 Internal medicine 、 Bone remodeling 、 Antiserum
摘要: We have reported the existence of specific and high-affinity calcitonin (CT) receptors on normal human T-lymphocytes. Because increasingly recognized importance interleukin-1 (IL-1) IL-6 in control bone metabolism, we examined their influence binding parameters labeled salmon (sCT) lymphocytes. After a 24-hour incubation, IL-1 at 100–5000 U/ml IL-6, 1–1000 U/ml, decreased apparent number CT sites (Bmax) The effects purified T-lymphocytes were dose related 100 U IL-6/ml reduced sCT to 57±16% (mean ± SD) values (n=6). There was no significant change affinity (Kd, 0.71±0.54x10-10 M for controls versus 0.90±0.55x10-10 after IL-6) decrease Bmax reversible 48 hours. appeared be mediated through an increased production as they neutralized by polyclonal antiserum against IL-6. Added alone, caused slight increase 115±5% (n=3). In summary, can induce marked loss concentrations known active metabolism. contributions our observations osteolytic activity these cytokines deserve further investigation.
ulb.ac.be
springer.com
sci-hub.se 参考文章(41)
L Nguyen, P V Hauschka, P Stashenko, F E Dewhirst, Interleukin-1 beta stimulates bone resorption and inhibits bone formation in vivo. Lymphokine and cytokine research. ,vol. 10, pp. 15- 21 ,(1991)
ARMEN H. TASHJIAN, DONALD R. WRIGHT, JOEL L. IVEY, ALLAN PONT, Calcitonin binding sites in bone: relationships to biological response and "escape". Recent Progress in Hormone Research. ,vol. 34, pp. 285- 334 ,(1978) , 10.1016/B978-0-12-571134-0.50012-0
H. Mulder, H. van Bolhuis, M. A. B. Naafs, P. L. M. Winckers, Influence of pharmacological doses of calcitonin on serum β2 microglobulin concentrationmicroglobulin concentration Calcified Tissue International. ,vol. 37, pp. 367- 371 ,(1985) , 10.1007/BF02553703
H Zola, L Flego, Expression of interleukin-6 receptor on blood lymphocytes without in vitro activation. Immunology. ,vol. 76, pp. 338- 340 ,(1992)
A Vecchi, E Dejana, A Biondi, J Van Damme, M Sironi, P Proserpio, A Mantovani, F Breviario, Il-1 stimulates il-6 production in endothelial cells Journal of Immunology. ,vol. 142, pp. 549- 553 ,(1989)
H G Remold, Y H Nong, R G Titus, J M Ribeiro, Peptides encoded by the calcitonin gene inhibit macrophage function. Journal of Immunology. ,vol. 143, pp. 45- 49 ,(1989)
S Humphreys, J P McGillis, S Reid, Characterization of functional calcitonin gene-related peptide receptors on rat lymphocytes. Journal of Immunology. ,vol. 147, pp. 3482- 3489 ,(1991)
Jacques Deviere, Jean Content, Chantal Denys, Paul Vandenbussche, Olivier Le Moine, Liliane Schandene, Jean-Pierre Vaerman, Etienne Dupont, Immunoglobulin A and interleukin 6 form a positive secretory feedback loop: A study of normal subjects and alcoholic cirrhotics Gastroenterology. ,vol. 103, pp. 1296- 1301 ,(1992) , 10.1016/0016-5085(92)91519-A
Karen Black, I. Ross Garrett, Gregory R. Mundy, Chinese Hamster Ovarian Cells Transfected with the Murine lnterleukin-6 Gene cause Hypercalcemia as well as Cachexia, Leukocytosis and Thrombocytosis in Tumor-Bearing Nude Mice Endocrinology. ,vol. 128, pp. 2657- 2659 ,(1991) , 10.1210/ENDO-128-5-2657
J. J. BODY, F. GLIBERT, S. NEJAI, G. FERNANDEZ, A. VAN LANGENDONCK, A. BORKOWSKI, Calcitonin Receptors on Circulating Normal Human Lymphocytes The Journal of Clinical Endocrinology and Metabolism. ,vol. 71, pp. 675- 681 ,(1990) , 10.1210/JCEM-71-3-675