Genetic Transformation of Amylase Gene to Ruminal Bacteroides Species Using Conjugation Consequence for Improvement of Rumen Enzyme
作者： G. Rahimi , F. Moradian , H. Rahimian , H. Oghbatalab
DOI:
关键词: Bacteroides 、 Shuttle vector 、 Escherichia 、 Biochemistry 、 Escherichia coli 、 Amylase 、 Expression vector 、 Bacteria 、 Microbiology 、 Biology 、 Rumen
摘要: Rumen bacterial strains can potentially be manipulated to perform functions different from wild type species. The most numerous species of bacteria in the rumen and gut are familyBacteroidetes, whichcan have potential for genetic modification enzyme production. One manipulation production starch digestive enhancement nutrient flow rumen. In this study, Bacteroides were isolated cows. 16S rRNA gene analysis was used confirm classification species. amylase Bacillus spp. cloned into expression vector pET28A then subcloned Escherichia coli-Bacteroides pGFK114.1 shuttle vector. Conjugation between coli a strainwas accomplished with two donors containing pRK231 one strain as recipient. Transfer by confirmed using marker cefoxcitin antibiotic resistant gene. activity assay showed that successful occurred ruminal
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