Sulforhodamine B Assay and Chemosensitivity

摘要: The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of protein dye bind electrostatically pH dependent basic amino acid residues trichloroacetic acid-fixed cells. Under mild acidic conditions it binds under can be extracted from cells solubilized measurement. Results SRB were linear with number cellular measured at densities ranging 1 200% confluence. sensitivity comparable that several fluorescence assays superior Lowry or Bradford. signal-to-noise ratio favorable resolution 1000-2000 cells/well. It performed similarly compared other such as MTT clonogenic assay. possesses a colorimetric end point nondestructive indefinitely stable. These practical advances make an appropriate sensitive even application.