Peptide enrichment by microfluidic electrocapture for online analysis by electrospray mass spectrometry.
作者： Susanne Vollmer , Juan Astorga-Wells , Gunvor Alvelius , Tomas Bergman , Hans Jörnvall
关键词: Electrospray ionization 、 Detection limit 、 Protein mass spectrometry 、 Chemical binding 、 Chromatography 、 Extractive electrospray ionization 、 Chemistry 、 Mass spectrometry 、 Sample preparation in mass spectrometry 、 Electrospray
摘要: Identification of peptides from a complex mixture can be difficult because the wide concentration range and different ionization efficiencies during analysis by electrospray (ESI) mass spectrometry (MS). Preconcentration methods are necessary to allow low-abundance low-intensity reach threshold spectrometer. Here we demonstrate peptide enrichment based on electroimmobilization. Peptides immobilized without use solid support or chemical binding application an electric field along microflow stream in electrocapture cell. Once enriched/preconcentrated inside cell, they released removal via interface with emitter submitted online spectrometric analysis. Tandem containing hemoglobin, myoglobin, bovine serum albumin (BSA), cytochrome c was successful. Amplification factors up 16-fold were achieved improvement signal-to-noise values for preconcentrated sample. The limit detection one 3.6 fmol.
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