Factorially designed crystallization trials of the full-length P0 myelin membrane glycoprotein. I. Precipitation diagram
作者： Jan Sedzik , Yoshiko Kotake , Keiichi Uyemura , Mitsuo Ataka
DOI: 10.1016/S0022-0248(02)01912-7
关键词: Single crystal 、 Protein crystallization 、 Supersaturation 、 Precipitation (chemistry) 、 Thermodynamics 、 Crystallography 、 Crystallization 、 Nucleation 、 Solubility 、 Crystal growth 、 Chemistry
摘要: P0 glycoprotein is the abundant membrane protein of myelin peripheral nervous system. We report now statistical design crystallization experiments; based on our belief that important information regarding supersaturation or its solubility nature, as well metastable state, nucleation precipitation, are hidden in trials which no crystals grow. It possible to work out this when whole set experiments designed such a way allow analyses. selected seven factors, we believe be for crystallization: concentration, pH buffer, nature precipitant, concentration detergent, additives and temperature. The experimental matrix detailed sheet make 148 solutions having random but balanced combination these levels were calculated using program DESIGN. A visual evaluation drops was performed light microscopy. able plot precipitation boundary diagram. Based diagram have eliminated factors (and levels) driving into precipitation. known related curves crystals, neighborhood further most likely occur. These conditions currently being refined identify (or can crucial obtaining large diffracting crystals. Full-length has never been crystallized structural determination.
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