Electron microscopic observations on healing in dental pulp in the rat

摘要: Abstract The process of repair in rat incisor dental pulp has been studied 150 animals with light and electron microscopy over a period 14 days following exposure capping calcium hydroxide. Within the zone injury created by operative procedure, inflammatory cells were present until fifth post-operative day. had commenced second day mitotic proliferation surrounding wound, next 2 many these migrated into displaying considerable morphological differentiation. Collagen fibrils appeared within wound on third day, fourth an extensive meshwork irregularly arranged was spaces between cells. In sections undecalcified tissue, small clusters crystals observed intimate relationship to collagen at various sites throughout wound. With progressive deposition crystals, large expanses calcified material uniform appearance formed. Decalcified revealed demineralized substructure crystal aggregates. earliest foci took form spherical structures less than μ dia., having amorphous electron-lucent peripheral around electron-dense central region. By numbers Growth fusion globules produced irregular masses which continued expand growth dark region, layer remaining approximately same width. again became visible, resembled mature dentine.