Whole-genome analysis of the SHORT-ROOT developmental pathway in Arabidopsis.

摘要: Stem cell function during organogenesis is a key issue in developmental biology. The transcription factor SHORT-ROOT (SHR) critical component pathway regulating both the specification of root stem niche and differentiation potential subset cells Arabidopsis root. To obtain comprehensive view SHR pathway, we used statistical method called meta-analysis to combine results several microarray experiments measuring changes global expression profiles after modulating activity. Meta-analysis was first identify direct targets by combining from an inducible form driven its endogenous promoter, ectopic expression, followed sorting comparisons mutant wild-type roots. Eight putative were identified, all with patterns encompassing subsets native domain. Further evidence for regulation came binding vivo promoter regions four eight targets. A new role vascular cylinder predicted pattern confirmed independent markers. approach then perform survey indirect Our analysis suggests that regulates development not only through large regulatory network but also hormonal pathways signaling using receptor-like kinases. Taken together, our nodes tissue reveal architecture this pathway.