Enhancing activity of artesunate against breast cancer cells via induced-apoptosis pathway by loading into lipid carriers
作者： Tuan Hiep Tran , Anh Ngoc Nguyen , Jong Oh Kim , Chul Soon Yong , Chien Ngoc Nguyen
DOI: 10.3109/21691401.2015.1129616
关键词: Apoptosis 、 Homogenization (biology) 、 Biology 、 Artesunate 、 Cancer 、 Biochemistry 、 Flow cytometry 、 Molecular biology 、 Sonication 、 Liposome 、 Staining
摘要: Artesunate-loaded nanostructured lipid carriers (ART-NLCs) were prepared by hot homogenization followed ultrasonication technique. The optimized ART-NLC demonstrated a particle size of 117.5 ± 6.1 nm, with good stability regarding zeta-potential of -19.47 ± 0.9 mV and drug entrapment efficiency 92.93 ± 1.47%. showed cellular uptake in breast cancer cells, which was confirmed confocal laser scanning microscopy (CLSM) flow cytometry analysis. significantly higher vitro cytotoxicity ART-NLCs against human MCF-7, MDA-MB-231 cells as compared the free ART recorded. Hoechst 33342 staining indicated that induced apoptosis rates MCF-7 well MDA-MB-231cells than ART.
sci-hub.se 参考文章(27)
Mohammad H. Darvishi, Alireza Nomani, Mohsen Amini, Mohammad A. Shokrgozar, Rassoul Dinarvand, Novel biotinylated chitosan-graft-polyethyleneimine copolymer as a targeted non-viral vector for anti-EGF receptor siRNA delivery in cancer cells International Journal of Pharmaceutics. ,vol. 456, pp. 408- 416 ,(2013) , 10.1016/J.IJPHARM.2013.08.069
Samuel Vidal Mussi, Renata Carvalho Silva, Mônica Cristina de Oliveira, Carolina Madeira Lucci, Ricardo Bentes de Azevedo, Lucas Antônio Miranda Ferreira, New approach to improve encapsulation and antitumor activity of doxorubicin loaded in solid lipid nanoparticles. European Journal of Pharmaceutical Sciences. ,vol. 48, pp. 282- 290 ,(2013) , 10.1016/J.EJPS.2012.10.025
Vladimir Torchilin, Tumor delivery of macromolecular drugs based on the EPR effect Advanced Drug Delivery Reviews. ,vol. 63, pp. 131- 135 ,(2011) , 10.1016/J.ADDR.2010.03.011
Thiruganesh Ramasamy, Ziyad S. Haidar, Tuan Hiep Tran, Ju Yeon Choi, Jee-Heon Jeong, Beom Soo Shin, Han-Gon Choi, Chul Soon Yong, Jong Oh Kim, Layer-by-layer assembly of liposomal nanoparticles with PEGylated polyelectrolytes enhances systemic delivery of multiple anticancer drugs Acta Biomaterialia. ,vol. 10, pp. 5116- 5127 ,(2014) , 10.1016/J.ACTBIO.2014.08.021
S. Martins, S. Costa-Lima, T. Carneiro, A. Cordeiro-da-Silva, E.B. Souto, D.C. Ferreira, Solid lipid nanoparticles as intracellular drug transporters: an investigation of the uptake mechanism and pathway. International Journal of Pharmaceutics. ,vol. 430, pp. 216- 227 ,(2012) , 10.1016/J.IJPHARM.2012.03.032
Anne Hamacher-Brady, Henning A. Stein, Simon Turschner, Ina Toegel, Rodrigo Mora, Nina Jennewein, Thomas Efferth, Roland Eils, Nathan R. Brady, Artesunate Activates Mitochondrial Apoptosis in Breast Cancer Cells via Iron-catalyzed Lysosomal Reactive Oxygen Species Production Journal of Biological Chemistry. ,vol. 286, pp. 6587- 6601 ,(2011) , 10.1074/JBC.M110.210047
Renu Chadha, Sushma Gupta, Natasha Pathak, Artesunate-loaded chitosan/lecithin nanoparticles: Preparation, characterization, and in vivo studies Drug Development and Industrial Pharmacy. ,vol. 38, pp. 1538- 1546 ,(2012) , 10.3109/03639045.2012.658812
Hudie Xie, Bo Yang, Fen Wang, Yulin Zhao, Host–guest inclusion system of artesunate with β-cyclodextrin and its derivatives: Characterization and antitumor activity Journal of Molecular Structure. ,vol. 1085, pp. 90- 96 ,(2015) , 10.1016/J.MOLSTRUC.2014.12.087
M Gabriëls, Physical and chemical evaluation of liposomes, containing artesunate. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 31, pp. 655- 667 ,(2003) , 10.1016/S0731-7085(02)00678-7
Thiruganesh Ramasamy, Tuan Hiep Tran, Ju Yeon Choi, Hyuk Jun Cho, Jeong Hwan Kim, Chul Soon Yong, ZS Haidar, Han-Gon Choi, Jong Oh Kim, Layer-by-layer coated lipid-polymer hybrid nanoparticles designed for use in anticancer drug delivery. Carbohydrate Polymers. ,vol. 102, pp. 653- 661 ,(2014) , 10.1016/J.CARBPOL.2013.11.009