HUMAN PANCREAS SCAFFOLDS
作者： Pinzani Massimo , De Coppi Paolo , Malago Massimo , Mazza Giuseppe , Al-Akkad Walid
DOI:
关键词: Human liver 、 Protease 、 Human pancreas 、 Cell biology 、 Liver tissue 、 Extracellular matrix 、 Osmotic shock 、 Disease modelling 、 Chemistry 、 Scaffold
摘要: This invention relates to methods for decellularising human liver tissue produce hepatic extracellular matrix (ECM) scaffolds, example use in therapy or disease modelling. The involve mechanically damaging cells the tissue, by freeze thaw, and then subjecting multiple cycles of osmotic stress, detergent treatment protease and/or DNAase a decellularised ECM scaffold.
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