Identification of bacteria associated with underground parts of Crocus sativus by 16S rRNA gene targeted metagenomic approach.
作者： Sheetal Ambardar , Naseer Sangwan , A. Manjula , J. Rajendhran , P. Gunasekaran
DOI: 10.1007/S11274-014-1694-0
关键词: Bulk soil 、 Pseudomonas 、 Rhizosphere 、 Crocus sativus 、 Botany 、 16S ribosomal RNA 、 Metagenomics 、 Acidobacteria 、 Biology 、 Bacteria
摘要: Saffron (Crocus sativus L), an autumn-flowering perennial sterile plant, reproduces vegetatively by underground corms. has biannual corm–root cycle that makes it interesting candidate to study microbial dynamics in its rhizosphere and cormosphere (area under influence of corm). Culture independent 16S rRNA gene metagenomic during flowering stage revealed presence 22 genera but none the genus was common all three samples. Bulk soil bacterial community represented 13 with Acidobacteria being dominant. In rhizosphere, out eight different identified, Pseudomonas most dominant genus. Cormosphere bacteria comprised six genera, dominated Pantoea. This composition samples is significantly (P < 0.05) from each other. first report on identification associated bulk Saffron, using cultivation 16S rRNA targeted approach.
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C. K. Shirkot, Anjali Chauhan, Rishi Mahajan, Preeti Mehta, P. K. Mahajan, Strain of Bacillus circulans isolated from apple rhizosphere showing plant growth promoting potential. Current Science. ,vol. 98, pp. 538- 542 ,(2010)
Patrick Wechter, Joey Williamson, Alison Robertson, Daniel Kluepfel, A rapid, cost-effective procedure for the extraction of microbial DNA from soil World Journal of Microbiology & Biotechnology. ,vol. 19, pp. 85- 91 ,(2003) , 10.1023/A:1022587806945
Tanushree Mazumdar, N. C. Talukdar, C. Goswami, Characterization and screening of beneficial bacteria obtained on King's B agar from tea rhizosphere. Indian Journal of Biotechnology. ,vol. 6, pp. 490- 494 ,(2007)
T A Hall, BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT Nucleic Acids Symposium Series. ,vol. 41, pp. 95- 98 ,(1999) , 10.14601/PHYTOPATHOL_MEDITERR-14998U1.29
Janaina F Araujo, Alinne P de Castro, Marcos MC Costa, Roberto C Togawa, Georgios J Pappas Júnior, Betania F Quirino, Mercedes MC Bustamante, Lynn Williamson, Jo Handelsman, Ricardo H Krüger, None, Characterization of Soil Bacterial Assemblies in Brazilian Savanna-Like Vegetation Reveals Acidobacteria Dominance Microbial Ecology. ,vol. 64, pp. 760- 770 ,(2012) , 10.1007/S00248-012-0057-3
Linda S. Jung, Silvia Winter, R. Lutz Eckstein, Monika Kriechbaum, Gerhard Karrer, Erik Welk, Martin Elsässer, Tobias W. Donath, Annette Otte, Colchicum autumnale L. Perspectives in Plant Ecology Evolution and Systematics. ,vol. 13, pp. 227- 244 ,(2011) , 10.1016/J.PPEES.2011.04.001
SunHee Hong, John Bunge, Chesley Leslin, Sunok Jeon, Slava S Epstein, Polymerase chain reaction primers miss half of rRNA microbial diversity. The ISME Journal. ,vol. 3, pp. 1365- 1373 ,(2009) , 10.1038/ISMEJ.2009.89
Aradhana Mishra, Puneet Singh Chauhan, Vasvi Chaudhry, Manisha Tripathi, Chandra Shekhar Nautiyal, None, Rhizosphere competent Pantoea agglomerans enhances maize (Zea mays) and chickpea (Cicer arietinum L.) growth, without altering the rhizosphere functional diversity Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 100, pp. 405- 413 ,(2011) , 10.1007/S10482-011-9596-8
D. M. Hillis, J. J. Bull, An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis Systematic Biology. ,vol. 42, pp. 182- 192 ,(1993) , 10.1093/SYSBIO/42.2.182
Krishna Kumar, Natarajan Amaresan, Someshwar Bhagat, Kutthum Madhuri, Ramesh Chandra Srivastava, Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops World Journal of Microbiology & Biotechnology. ,vol. 27, pp. 1625- 1632 ,(2011) , 10.1007/S11274-010-0616-Z