Endothelium-dependent and -independent relaxation induced by pinocembrin in rat aortic rings.
作者： Xiao-Ming Zhu , Lian-Hua Fang , Yu-Juan Li , Guan-Hua Du
DOI: 10.1016/J.VPH.2006.09.003
关键词: Biophysics 、 Aorta 、 Glibenclamide 、 Tetraethylammonium 、 Contraction (grammar) 、 Stereochemistry 、 Methylene blue 、 Calcium 、 Chemistry 、 Pinocembrin 、 Nitric oxide
摘要: Abstract The aim of present study was to evaluate the vasorelaxant effects flavonone pinocembrin and its possible mechanisms in isolated rat aortic rings. Pinocembrin (5 ∼ 100 μM) induced relaxation rings pre-contracted with norepinephrine (NE, 1 μM) or KCl (60 mM), pEC50 value 4.37 ± 0.02 4.52 ± 0.04. Pretreatment (30 50 μM) also inhibited contractile responses NE KCl. effect relied on intact endothelium partially, incubation n ω-nitro- l -arginine methyl ester ( -NAME, 100 μM) methylene blue (10 μM) significantly effect, however indomethacin (5 μM) had no influence action. In endothelium-denuded rings, reduced by glibenclamide (10 μM), tetraethylammonium (5 mM) 4-aminopyridine (100 μM). NE-induced transient contraction Ca2+-free solution increasing external calcium medium plus 60 mM Our results suggest that induces through an endothelium-dependent pathway, involving NO-cGMP, endothelium-independent opening K+ channels blockade Ca2+ channels.
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