Sephadex LH-20 multiple-column chromatography for the simultaneous separation of progesterone, deoxycorticosterone and 17α-hydroxyprogesterone from small plasma samples
作者： W.A. Golder , W.G. Sippell
DOI: 10.1016/S0021-9673(00)82197-4
关键词: Solvent 、 Plasma samples 、 Elution 、 Chromatography 、 Solvent system 、 Column chromatography 、 Ethanol 、 17α-Hydroxyprogesterone 、 Sephadex 、 Chemistry
摘要: Abstract A simple and convenient chromatographic method is described for the simultaneous complete separation of unconjugated steroids progesterone, deoxycorticosterone 17α-hydroxyprogesterone on 40-cm columns Sephadex LH-20 using a water-saturated solvent system containing n-heptane-chloroform (1:1) plus 0.25% ethanol. Manual operation up to ten run in parallel facilitated by use graduated, cylindrical, reservoirs top columns. Thus, negligible column-to-column, only limited day-to-day, variations occur elution patterns, constant high recoveries are obtained. When combined with previously 60-cm chromatography, eight important corticosteroids can be isolated individually from 16 20 small plasma samples per day.
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