Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper

摘要: Bulked segregant analysis (BSA) using Affymetrix GeneChips revealed candidate genes underlying the major QTL for Phytophthora capsici resistance in Capsicum . Using genes, reliable markers were developed and validated. Phytophthora capsici L. is one of most destructive pathogens pepper (Capsicum spp.). Resistance against P. controlled by quantitative trait loci (QTL), including a on chromosome 5 that predominant contributor to resistance. Here, maximize effect this study its an F2 population recombinant inbred lines inoculated with strain JHAI1-7 zoospores at low concentration (3 × 103/mL). phenotype segregation ratios for populations fit 3:1 1:1 (resistant:susceptible) model, respectively, consistent single dominant gene model. position polymorphism (SPP) marker mapping QTL. When SPP (Phyto5SAR) together other SNP located was used confirm QTL, Phyto5SAR showed highest LOD value A scaffold sequence (scaffold194) containing identified from C. annuum genome database. The contained two putative NBS-LRR SAR 8.2A as candidates contributing Markers linked these validated testing 100 F1 commercial cultivars. Among markers, Phyto5NBS1 about 90 % accuracy in predicting phenotypes low-virulence isolate. These results suggest can be identification gene(s) 5.