Processing of exogenous heat-aggregated (denatured) and particulate (native) hepatitis B surface antigen for class I-restricted epitope presentation.

摘要: Many cell types efficiently present an epitope of the hepatitis B surface Ag (HBsAg) to murine class I-restricted CTL following in vitro pulse with native 22-nm HBsAg particles. Processing exogenous particles required its cytochalasin B-insensitive uptake and acid proteolysis endocytic compartment, was insensitive brefeldin A cycloheximide, did not involve regurgitation antigenic peptides. In contrast, after cells exogenous, heat-denatured 1-micron aggregates, only macrophages (but other tested) presented Ld-restricted CTL. aggregates B-sensitive uptake, A, involved two different, preparations for presentation thus alternative pathways: "endocytic pathway" particles, a "phagocytic denatured 1-microns aggregates. Both displayed different immunogenicity vivo when delivered without adjuvants: were high immunogenicity, low immunogenicity. Class are primed processing" as well