Syntaxin-11, but not syntaxin-2 or syntaxin-4, is required for platelet secretion

摘要: The platelet release reaction plays a critical role in thrombosis and contributes to the events that follow hemostasis. Previous studies have shown secretion is mediated by Soluble NSF Attachment Protein Receptor (SNARE) proteins from granule plasma membranes. SNAREs form transmembrane complexes mediate membrane fusion cargo release. Although VAMP-8 (v-SNARE) SNAP-23 (a t-SNARE class) are important for secretion, identity of functional syntaxin (another has been controversial. using anti-syntaxin Abs permeabilized platelets suggested roles both syntaxin-2 syntaxin-4. In present study, we tested these conclusions syntaxin-knockout mouse strains Familial Hemophagocytic Lymphohistiocytosis type 4 (FHL4) patient. Platelets syntaxin-4 single- or double-knockout mice had no defect. FHL4 patient deficient syntaxin-11 robust defect agonist-induced although their morphology, activation, levels appeared normal. Semiquantitative Western blotting showed more abundant human murine platelets. Coimmunoprecipitation experiments can SNARE with SNAP-23. results study indicate syntaxin-11, but not syntaxin-4, required exocytosis.