Aptamer-based spectrofluorometry for cellular prion protein using N,N′-bis[3,3′-(dimethylamino)propylamine]-3,4,9,10-perylenetetracarboxylic diimide

摘要: A new spectrofluorometric method for cellular prion protein (PrPC) was developed based on the regulation of N,N′-bis[3,3′-(dimethylamino)propylamine]-3,4,9,10-perylenetetracarboxylic diimide (DAPER) fluorescence. As a perylene derivative, DAPER emits strong fluorescence in form free monomer aqueous medium, but not aggregates. In this contribution, we found that aptamer PrPC could induce aggregation DAPER, and bright completely quenched. The quenched fluorescence, however, recovered if further added, which ascribed to specific binding its releasing monomers. This signalling mechanism makes it possible detect by spectroscopy. assay allows selective determination solution with high sensitivity exhibits good linear range from 0.4 1.6 nmol L−1. Moreover, probe can be applied monitor level human urine samples satisfactory results.