STAT3 activates the transcription of lncRNA NR2F1‐AS1 to promote progression of melanoma via regulating miR‐493‐5p/GOLM1 axis

摘要: BACKGROUND Long non-coding RNAs (lncRNAs) are vital regulators during the biological processes of melanoma. The present study aimed to uncover functions lncRNA termed NR2F1 antisense RNA 1 (NR2F1-AS1) in melanoma and potential mechanisms. METHODS Relative levels NR2F1-AS1 miR-493-5p a total 137 paired primary tissues corresponding non-tumor tissues, as well three cell lines, were examined by real-time polymerase chain reaction. clinical significance expression was analyzed statistically. STAT3 binding motif promoter region identified JASPAR (http://jaspar.genereg.net). association between determined dual-luciferase reporter chromatin immunoprecipitation assays. effects on proliferation, migration measured counting kit-8 (CCK-8), Edu, transwell wound healing Dual-luciferase pull-down assays applied validate interaction among NR2F1-AS1, GOLM1. Furthermore, vivo experiments conducted demonstrate oncogenic role RESULTS Up-regulated down-regulated detected tumors cells. overexpression induced STAT3. High correlated advanced tumor stage poor prognosis Functional studies using CCK-8, revealed that proliferative, migratory invasive capacities cells attenuated inhibition NR2F1-AS1. Moreover, able up-regulate GOLM1 through recognizing miR-493-5p. knockdown distinctly reversed these inhibitory down-regulation tumorigenesis progression CONCLUSIONS Our findings key for via targeting miR-493-5p/GOLM1 axis.