Long-lived Intermediates in Phytochrome Transformation II: In Vitro and In Vivo Studies

摘要: Conditions of illumination which cause phytochrome to cycle rapidly from P(R) P(FR) and back lead the accumulation in vivo detectable amounts long-lived intermediates on pathway oat coleoptile tissue. They appear decay independently parallel P(FR). Their behavior under different intensities exposure time suggests that they are homologous with 2 similar previously observed vitro. Available evidence favoring this suggestion is discussed. Equivalent apparently causes far higher steady state levels absorption by than vitro, native a physical cell it solution. A difference spectrum for vitro between 365 580 nm presented. It has maximum at 380 nm, minimum 418 crossover points 398 485 nm. Glycerol sample enhances signal without otherwise changing any way. The represents combined