Pressurized liquid extraction of pigments from Chlamydomonas sp. and chemical characterization by HPLC-MS/MS
作者： Lidia Montero , Mahshid Sedghi , Yolanda García , Carlos Almeida , Carl Safi
DOI: 10.1007/S41664-018-0062-8
关键词: Chlorophyll 、 Chlamydomonas 、 Pigment 、 Carotenoid 、 Yield (chemistry) 、 Extraction (chemistry) 、 Lutein 、 Chromatography 、 Chemistry 、 Antioxidant
摘要: Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed determine effect of conditions on yield antioxidant capacity different extracts determined 1,1-diphenyl-2-picryl-hydrazyl TEAC tests. All show significant activity in all assays. Besides, this work, carotenoid chlorophyll composition analyzed by HPLC–DAD–APCI–MS/MS for first time, being main compounds lutein pheophythin a´.
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